cDNA cloning and gene expression of the type 1 bovine interleukin-1 receptor

Regulation of interleukin-l (IL-1) mediated biological responses is complic ated by the multiple Ligands and receptors of the IL-1 family. Most studies of IL-1 receptors have used human or rodent cells. Here, we report that th e coding region of the bovine type 1 interleukin-l receptor (type 1 IL-1R) cDNA extends 1719 bp in length. Northern analysis of specific bovine cell a nd tissue RNA demonstrated a 4.5kb transcript. Overall, the bovine type 1 I L-1R coding region exhibits approximately 81 and 76% similarity with the hu man type 1 IL-1R at the nucleotide and amino acid level, respectively, and somewhat less similarity with the mouse acid rat sequences, Type 1 IL-1R tr anscripts were confirmed by RT-PCR in several bovine cell types, including peripheral blood mononuclear cells (PBMCs), neutrophils (PMNs), and fibrobl ast, peritoneal macrophage, and arterial endothelial cell lines. It is expe cted that molecular clones for the bovine type 1 and 2 IL-1 receptors will provide us with the tools needed to decipher species-and cell-specific regu lation of IL-1 action in the bovine. (C) 1999 Elsevier Science B.V. All rig hts reserved.