Influence of So/Xo ratio on anaerobic activity test

The effect of the substrate/microorganism ratio during the development of a naerobic activity test was studied. The experimentation was carried out in serum bottles at 35 degrees C. Two sets of experiments utilizing acetate an d an azo dye (blue disperse 79) as the sole source of carbon were studied. It was observed that mixing has an important influence on the results. The initial substrate concentration and the initial biomass concentration had a significant effect on the reaction rate and on the biomass yield coefficie nt. Yobs. Different kinetic coefficients were found for the case of equal S o/Xo ratio, but different initial substrate concentration. (C) 1999 Publish ed by Elsevier Science Ltd on behalf of the IAWQ. All rights reserved.