S. Echeverrigaray et al., Isolation and characterization of Metolachlor-resistant mutants of Saccharomyces cerevisiae, WORLD J MIC, 15(6), 1999, pp. 679-681
The herbicide Metolachlor (alpha-chloroacetamide group) inhibits the growth
of Saccharomyces cerevisiae on complete, minimal, and non-fermentative med
ia. Spontaneous and induced resistant mutants showed monogenic segregation
patterns. Among the resistant clones, 70% were recessives, 16.4% were parti
ally dominants and 13.4% were dominants. The spontaneous partially dominant
mutation Mtc1 was mapped on linkage group XV at 33.3 cM from ade2 and 31.7
cM from his3, in a region that is characterized by the presence of several
resistant genes. The recessive mutation mtc2 was located on chromosome IV.
Although all the mutants had the ability to grow in the presence of the he
rbicide, they remained affected in their respiration efficiency, indicating
two different mechanism of action of Metholachor on yeast cells.