Rosmarinic acid production by Lavandula vera MM cell suspension culture: nitrogen effect

The growth of Lavandula vera MM cell suspension and the biosynthesis of ros marinic acid (RA) were followed during its cultivation in Linsmayer-Skoog m edia, containing different concentrations of ammonium and nitrate ions. The results showed that cultivation in a medium with 0.09 g ammonium ions/l (1 /4 of standard medium) ensured intensive growth (16 g dry biomass/l) and en hanced biosynthesis of RA (15 mg/g dry biomass). Cultivation of L. vera MM cell culture in a medium with 1.2-fold concentration of nitrate ions led to accumulation of 11 mg RA/g dry biomass which was twice as much as in the s tandard Linsmayer-Skoog medium.