Citation
Sn. Zhou et al., Isolation and characterization of chitinase from a marine bacterium Vibriosp., WORLD J MIC, 15(6), 1999, pp. 745-746
Citations number
11
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
09593993
→ ACNP
Volume
15
Issue
6
Year of publication
1999
Pages
745 - 746
Database
ISI
SICI code
0959-3993(199912)15:6<745:IACOCF>2.0.ZU;2-U
Abstract
A chitinase was separated from the culture broth of Vibrio sp. 11211 isolat
ed from sediment from the South China Sea. The chitinase was purified 18.3-
fold with 33% recovery by ammonium sulphate precipitation and chromatograph
y. The subunit molecular weight of the enzyme was estimated by SDS-PAGE to
be about 30 kDa. The enzyme showed optimum pH at 6.5 and optimum temperatur
e at 50 degrees C, and was stable in the pH range of 4 to 9 and at the temp
erature below 40 degrees C.