CHIRAL SEPARATION OF DRUGS BY CAPILLARY ELECTROPHORESIS USING BETA-CYCLODEXTRIN POLYMER

The direct separation of enantiomers of trimetoquinol hydrochloride, r elated substances and some other drugs was investigated by capillary e lectrophoresis employing six kinds of cyclodextrins (CDs). Enantiomeri c recognition of trimetoquinol and related substances was successfully achieved by using beta-cyclodextrin (beta-CD), heptakis(2,6- di-O-met hyl)-beta-cyclodextrin (DM-beta-CD) and beta-CD polymer. beta-CD polym er was especially effective for the separation of both enantiomers and different solutes. The effects of the amount of beta-CD polymer added to the background electrolyte, the pH of the buffer solution and some additives such as an organic solvent or a surfactant on the resolutio n of enantiomers were examined. The best enantioseparation was obtaine d by employing 5-7% beta-CD polymer in an acidic solution with a surfa ctant.