Crystallization of Helix pomatia agglutinin (HPA), a protein from the edible snail

Authors
Lisgarten, JN Pitts, JE Palmer, RA Reynolds, CD Dao-Thi, MH Van Driessche, E Beeckmans, S
Citation
Jn. Lisgarten et al., Crystallization of Helix pomatia agglutinin (HPA), a protein from the edible snail, ACT CRYST D, 55, 1999, pp. 1903-1905
Citations number
19
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
11
Pages
1903 - 1905
Database
ISI
SICI code
0907-4449(199911)55:<1903:COHPA(>2.0.ZU;2-P
Abstract
Crystals of Helix pomatia agglutinin (HPA) have been grown by the hanging-d rop technique using poryethylene glycol as the precipitant at 293 K. Over a period of one to two weeks the crystals grew to maximum dimensions of 0.10 x 0.05 x 0.02 mm. The crystals belong to space group P6(3)22, with unit-ce ll dimensions a = b = 63.3, c = 105.2 Angstrom and Z = 12 identical monomer s of M-r = 13 kDa, aggregating into two 78 kDa hexameric protein molecules per unit cell, each with symmetry 32 (D-3) The diffraction pattern extends to 3.6 Angstrom at 293 K.