Crystals of Helix pomatia agglutinin (HPA) have been grown by the hanging-d
rop technique using poryethylene glycol as the precipitant at 293 K. Over a
period of one to two weeks the crystals grew to maximum dimensions of 0.10
x 0.05 x 0.02 mm. The crystals belong to space group P6(3)22, with unit-ce
ll dimensions a = b = 63.3, c = 105.2 Angstrom and Z = 12 identical monomer
s of M-r = 13 kDa, aggregating into two 78 kDa hexameric protein molecules
per unit cell, each with symmetry 32 (D-3) The diffraction pattern extends
to 3.6 Angstrom at 293 K.