Induction of nitric oxide in bovine peripheral blood mononuclear cells by bovine herpesvirus 1

Bovine peripheral blood mononuclear cells (PBMCs) and monocytes were stimul ated with bovine herpesvirus 1 (BHV-1), LPS and concanavalinA (Con A) to pr oduce L-arginine-dependent nitric oxide (NO) in vitro. NO was detected as e arly as 12 hrs and up to 72 hrs post stimulation (p.s.). The NO from lipopo lysacharide (LPS)-stimulated PBMCs and monocytes was found to exhibit antiv iral effect against BHV-1. The anti-BHV-1 effect was inhibited with N-omega -methyl L-arginine indicating involvement of inducible NO synthase (iNOS) i n NO production.