EXPRESSION OF RECOMBINANT HUMAN CERULOPLASMIN - AN ABSOLUTE REQUIREMENT FOR SPLICING SIGNALS IN THE EXPRESSION CASSETTE

Authors
RAFIQ M SUEN CKM CHOUDHURY N JOANNOU CL WHITE KN EVANS RW
Citation
M. Rafiq et al., EXPRESSION OF RECOMBINANT HUMAN CERULOPLASMIN - AN ABSOLUTE REQUIREMENT FOR SPLICING SIGNALS IN THE EXPRESSION CASSETTE, FEBS letters, 407(2), 1997, pp. 132-136
Citations number
33
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
407
Issue
2
Year of publication
1997
Pages
132 - 136
Database
ISI
SICI code
0014-5793(1997)407:2<132:EORHC->2.0.ZU;2-V
Abstract
We report the successful expression of recombinant human ceruloplasmin which was made possible by inclusion of splicing signals in the expre ssion vector. Ceruloplasmin cDNA expressed from the vector pNUT in bab y hamster kidney cells gave protein yields of 0.03 mg/l which increase d to 15 mg/l with splicing signals present, The defect in expression f rom the intronless cDNA is due to complete retention of ceruloplasmin mRNA in cell nuclei. The block to cytoplasmic export is alleviated by splicing signals, allowing full expression of the mRNA. (C) 1997 Feder ation of European Biochemical Societies.