Diazene JK-279: potential anticancer drug

The aim of this study was to examine the cytotoxic effect of 10 newly synth esized diazenecarboxamides (diazenes). Using a modified colorimetric MTT as say, their cytotoxicity was determined on 10 human cell lines: cervical car cinoma parental and cisplatin-resistant cells, laryngeal carcinoma parental and cisplatin- and vincristine-resistant cells, glioblastoma parental and cisplatin-resistant cells, breast adenocarcinoma parental and doxorubicin-r esistant cells, and mammary carcinoma cells. Results show that diazene JK-2 79 was most effective, reducing significantly the cell survival of all 10 c ell lines examined, including five drug-resistant cell lines. A cytotoxic e ffect was observed also on nine from 10 cell lines for diazene JK-835. A sm all reduction in cell survival was obtained (mainly for highest drug concen trations) for diazenes LV-57 and MG-19 on two cell lines, and JK-429 and JK -913 on one cell line. Other diazenes did not demonstrate any cytotoxic act ivity. The results encourage further research on diazene JK-279 as a potent ial anticancer drug. [(C) 1999 Lippincott Williams & Wilkins.].