Expression of the csp protein family upon cold shock and production of tetracycline in Streptomyces aureofaciens

Citation
K. Mikulik et al., Expression of the csp protein family upon cold shock and production of tetracycline in Streptomyces aureofaciens, BIOC BIOP R, 265(2), 1999, pp. 305-310
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
265
Issue
2
Year of publication
1999
Pages
305 - 310
Database
ISI
SICI code
0006-291X(19991119)265:2<305:EOTCPF>2.0.ZU;2-J
Abstract
A shift down in temperature causes in Streptomyces aureofaciens a transient repression of polypeptide synthesis. During the acclimation phase 32 prote ins were synthesized. The addition of tetracycline (200 mu g/ml) to cells f rom exponential phase of growth leads to induction of 27 novel proteins and 17 upregulated proteins migrated in 2-D gel as proteins expressed upon col d shock. Immunoblot analysis using antibodies raised against CspB, CspC, an d CspD of Bacillus subtilis revealed five cross-reactive proteins of the Cs p family. Proteins CspB and CspD are predominantly induced at low temperatu re or by the presence of tetracycline. Expression of Csp proteins during th e acclimation phase is regulated on the transcription level. Proteins of th e Csp family have been shown to be associated with ribosomes and can be rem oved by 1 M. NH,CI. As expression of Csp proteins differs during developmen t or temperature shift down, these proteins can be considered as trans-acti ng factors to form contacts with the coding region of specific mRNAs. (C) 1 999 Academic Press.