Migration of CD18-deficient neutrophils in vitro: evidence for a CD18-independent pathway induced by IL-8

Neutrophils isolated from a child with severe leukocyte adhesion deficiency 1 (LAD1) had a complete absence of expression of the CD11/CD18 beta 2 inte grin family of adhesion molecules, and were shown to be deficient in the in vitro adhesion and migration properties. However, we found that interleuki n-8 (IL8), a potent chemoattractant for neutrophils, and sputum sol phase i nduced these LAD1 neutrophils to migrate through an endothelial cell layer in vitro, and confirmed that this migration was CD18-independent. These fin dings add to evidence of CD18-independent mechanisms of neutrophil recruitm ent, in particular neutrophil infiltration into the lungs, where IL8 may be an important recruitment factor. (C) 2000 Elsevier Science B.V. All rights reserved.