A. Miltner et W. Zech, Microbial degradation and resynthesis of proteins during incubation of beech leaf litter in the presence of mineral phases, BIOL FERT S, 30(1-2), 1999, pp. 48-51
Organic N constitutes more than 90% of total N in surface soil horizons. Am
ino acids, peptides and proteins represent the most abundant N species. The
re are indications that clay minerals influence the degradation of proteins
, but little is known about the effect of oxides and hydroxides on protein
decay. We therefore conducted an incubation experiment with mixtures of bee
ch leaf litter and Fe oxide, Al hydroxide, Mn oxide or quartz sand. The pro
tein amounts (quantified as alpha-NH2-N) during the 498-day experiment were
recorded. During the first 90-239 days, plant-derived proteins were decomp
osed, resulting in a decline of protein amounts to about 60% of the initial
value. Later in the experiment, the protein amounts increased again to bet
ween 70% and 90% of the initial amount, because microbial resynthesis of pr
oteins outweighed decomposition. The change from dominating decomposition t
o prevailing microbial resynthesis occurred when the microorganisms had to
adapt to less favourable conditions and therefore built new, protein-rich b
iomass. Although the mineral phases did not influence protein decomposition
initially, Fe oxide and Al hydroxide stabilized plant-derived proteins. Al
hydroxide reduced protein resynthesis in the second phase of the experimen
t, probably due to a reduction of microbial activity. Mn oxide increased pr
otein decomposition and lowered microbial resynthesis due to its oxidative
properties. The mineral phases therefore resulted in a shift of the relativ
e intensities of protein decomposition and microbial resynthesis.