Exploring the architecture of potassium channels using chimaeras to revealsignal transduction

Authors
Chanda, B Tiwari, JK Varshney, A Mathew, MK
Citation
B. Chanda et al., Exploring the architecture of potassium channels using chimaeras to revealsignal transduction, BIOSCI REP, 19(4), 1999, pp. 301-306
Citations number
12
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOSCIENCE REPORTS
ISSN journal
01448463 → ACNP
Volume
19
Issue
4
Year of publication
1999
Pages
301 - 306
Database
ISI
SICI code
0144-8463(199908)19:4<301:ETAOPC>2.0.ZU;2-F
Abstract
The chimaeric channel, 4N/1, generated from two outwardly rectifying Ki cha nnels by linking the N-terminal cytoplasmic domain of hKv1.4 with the trans membrane body of hKv1.4, functions as an inward rectifier. The operating ra nge of the channel is shifted to hyperpolarizing potentials and it is inact ivated at resting membrane potentials. Coexpression of a truncated form of hKv1.1 with the N-terminal domain of hKv1.4 results in the same physiology as the chimaera implying specific interactions between the two segments.