Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive

Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for fe ed enzyme were compared on the basis of phosphate inhibition and thermal st abilities. The apparent half-life of the former enzyme at 80 degrees C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 m M phosphate while 3 mM phosphate inhibited 50% of the original activity in case of A. ficuum phytase. Addition of 5 mM CaCl2 significantly broadened t he active pH range and also increased the pH stability of DS11 phytase.