Synthesis of homo- and hetero-oligosaccharides by Thai rosewood beta-glucosidase

Authors
Srisomsap, C Subhasitanont, P Techasakul, S Surarit, R Svasti, J
Citation
C. Srisomsap et al., Synthesis of homo- and hetero-oligosaccharides by Thai rosewood beta-glucosidase, BIOTECH LET, 21(11), 1999, pp. 947-951
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
11
Year of publication
1999
Pages
947 - 951
Database
ISI
SICI code
0141-5492(199911)21:11<947:SOHAHB>2.0.ZU;2-T
Abstract
beta-Glucosidase from Thai Rosewood (Dalbergia cochinchinensis Pierre) cata lyzes the synthesis disaccharides and trisaccharides at 40-70% (w/w) glucos e. Gentiobiose (beta 1-6-diglucose) was the major disaccharide. The reactio n was optimal at pH 5.0 and 60-65 degrees C. When the reaction was performe d in mixtures of glucose and fucose, a novel product was found, which was p urified and characterized by NMR spectroscopy to be d-fucosyl (beta 1-6)-d- glucose.