H. Fukuda et Y. Kizaki, A new transformation system of Saccharomyces cerevisiae with blasticidin Sdeaminase gene, BIOTECH LET, 21(11), 1999, pp. 969-971
A new method for transformation of Saccharomyces cerevisiae that allows sel
ection was developed. As the frequency of spontaneous blasticidin S resista
nt mutants from diploid type yeast strain (X-2180AB) was 5.2x10(-6), which
was a thousandfold less than that from haploid type yeast strain (X-2180B),
it was considered that the mechanism of spontaneous blasticidin S resistan
t mutations was related to recessive gene. Industrial yeasts, which were di
ploid, were transformed with blasticidin S deaminase gene from Aspergillus
terreus to blasticidin S resistance. Expression of blasticidin S deaminase
gene allowed selection of transformants from industrial yeasts.