High level expression of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator in silkworm

Authors
Peng, R Yu, ZY Zang, YH Qin, JC
Citation
R. Peng et al., High level expression of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator in silkworm, BIOTECH LET, 21(11), 1999, pp. 979-985
Citations number
20
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
11
Year of publication
1999
Pages
979 - 985
Database
ISI
SICI code
0141-5492(199911)21:11<979:HLEOAM>2.0.ZU;2-A
Abstract
A cDNA of a mutant (K151E, R154G) of single chain urokinase-type plasminoge n activator (mscu-PA) was constructed to include the natural scu-PA signal peptide sequences and transferred into the genome of Bombyx mori nuclear po lyhedrosis virus (BmNPV) by transfer vectors pBE284 (derived from BmNPV) an d pVL1392 (from AcNPV), respectively. Both Bombyx mori (BmN) cells and silk worm larvae were infected with the two recombinant viruses. Fibrin-plate as say showed that the re-virus from pVL1392 increased the yield of mscu-PA th ree times compared with the re-virus from pBE284.