Citation
Pjl. Bell et al., Rapid cloning of thermoalkalophilic lipases from Bacillus spp. using PCR, BIOTECH LET, 21(11), 1999, pp. 1003-1006
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492
→ ACNP
Volume
21
Issue
11
Year of publication
1999
Pages
1003 - 1006
Database
ISI
SICI code
0141-5492(199911)21:11<1003:RCOTLF>2.0.ZU;2-D
Abstract
A lipase gene from a thermophilic Bacillus sp. TG43, whose product showed o
ptimal activity at alkaline pH, was cloned using a lambda expression librar
y. Consensus PCR primers were designed based on a DNA sequence alignment of
the cloned lipase with two other homologous lipases reported in the litera
ture. The consensus primers allowed rapid cloning and expression of several
novel lipases from DNA of both pure cultures of Bacillus and biomass from
thermophilic environmental samples.