Progresses in separation and sensing technologies using DNA as affinity ligands

Authors
Citation
D. Umeno et M. Maeda, Progresses in separation and sensing technologies using DNA as affinity ligands, BUNSEKI KAG, 48(10), 1999, pp. 871-890
Citations number
256
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
BUNSEKI KAGAKU
ISSN journal
05251931 → ACNP
Volume
48
Issue
10
Year of publication
1999
Pages
871 - 890
Database
ISI
SICI code
0525-1931(199910)48:10<871:PISAST>2.0.ZU;2-L
Abstract
The recent developments of affinity techniques concerning single- and doubl e-stranded DNA are reviewed. Based on the opinion that the immobilization o f DNA is essential for the analytical and preparative use of the ligand fun ction of DNAs, the two items are extensively discussed concerning the choic e of conjugation chemistry and supports. Finally, a variety of applications , mainly for sensing and purification, are discussed.