T. Yoshida et al., Calculation of the amino acid hydrophilicity index for retention of peptides separation in a normal-phase LC, BUNSEKI KAG, 48(10), 1999, pp. 917-926
It was reported in a previous paper that the separation of peptides took pl
ace in normal-phase liquid chromatography on amide, diol and silica columns
. In this study, the contribution of individual amino acid residues to the
retention of peptides was evaluated in order to clarify the mechanism of pe
ptide separation. This paper lists the "hydrophilicity retention coefficien
ts". The retentions of 121 peptides were measured through normal-phase liqu
id chromatography on the amide, diol and silica columns using an acetonitri
le-water mixed solution containing 0.2% trifluoroacetic acid + 0.2% triethy
lamine as the mobile phase in a linear gradient elution system. For the sil
ica column, the best-fit hydrophilicity retention coefficients could be cal
culated by a linear-regression analysis. For the amide and diol columns, th
e best-fit hydrophilicity retention coefficients could be calculated by a n
on-linear regression analysis. A set of the hydrophilicity retention coeffi
cients on each column successfully explained the contribution degree of ret
ention on each column.