Post-column detection of isoluminol-labelled bile acids on bariumperoxide as chemiluminescence reaction media

Authors
Goto, H Lin, JM Yamada, M
Citation
H. Goto et al., Post-column detection of isoluminol-labelled bile acids on bariumperoxide as chemiluminescence reaction media, BUNSEKI KAG, 48(10), 1999, pp. 945-948
Citations number
4
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
BUNSEKI KAGAKU
ISSN journal
05251931 → ACNP
Volume
48
Issue
10
Year of publication
1999
Pages
945 - 948
Database
ISI
SICI code
0525-1931(199910)48:10<945:PDOIBA>2.0.ZU;2-K
Abstract
An isoluminol chemiluminescent(CL) reactin on bariumperoxide (BaO2) was app lied for the detection of bile acids after high performance liquid chromato graphic (HPLC) separation. N-(4-Aminobutyl)-N-ethylisoluminol (ABEI) was us ed as a pre-labelling reagent for bile acids. In the proposed CL detection system, no CL reagents were required, because superoxide existed on the bas ic surface of BaO2. Ursodeoxycholic acid was detected at levels as low as 1 0 pmol, more sensitive than that in HPLC-UV methods.