Induction of gastritis and gastric ulcer in Mongolian gerbils infected with Helicobacter pylori

Objective To induce the model of Mongolian gerbil infected by Helicobacter pylori (HP) and to observe the changes of the infiltration and the prolifer ation of inflammatory cells before and after the bactericidal treatment. Methods The animal model of HP infection was induced by inoculating the HP strain (American Type Culture Collection) to male Mongolian gerbil MGS/Sea (SPF). Half a month, 1 month and 3 months after inoculation, kill the anima ls and make HE staining and immunohistochemistry staining of the stomach to observe the pathological changes. Results The infiltration of a great number of inflammatory cells was observ ed with the center of the mucosa of pylorus at the second week of HP infect ion. At the 3rd month of infection, ulcer appeared near the lesser curvatur e side of the pylorus. On the early stage of infection, the lesion was acut e inflammation characterized by the neutrophilic infiltration, and then tra nsformed into the chronic inflammation characterized by the lymphatic infil tration and the wide formation of lymphatic follicles. Conclusion Mongolian gerbil is an ideal and standard animal model of HP inf ection.