Methylotrophic yeasts contain large peroxisomes during growth on methanol.
Upon exposure to excess glucose or ethanol these organelles are selectively
degraded by autophagy, Here we describe the cloning of a Pichia pastoris g
ene (PpVPS15) involved ill peroxisome degradation, which is homologous to S
accharomyces cerevisiae VPS15. In methanol-grown cells of a P. pastoris VPS
15 deletion strain, the levels of peroxisomal marker enzymes remained high
after addition of excess glucose or ethanol. Electron microscopic studies r
evealed that the organelles were not taken up by vacuoles, suggesting that
PpVPS15 is required at an early stage in peroxisome degradation.