Analysis of chiral drugs with enantioselective biosensors. An overview

The opportunity to use amperometric biosensors based on L-aminoacid oxidase in enantioselective analysis of chiral drugs is discussed. The designed bi osensors combine the selectivity of the enzyme with the sensitivity of the amperometric device. To obtain a reliable amperometric biosensor, graphite paste is used as support for the enzyme. The parameters obtained for ampero metric biosensors are compared with those obtained for the potentiometric e nantioselective membrane electrodes based on P-cyclodextrin derivates impre gnated in graphite paste for several angiotensin converting enzyme (ACE) in hibitors-S-captopril, S-enalapril, S-ramipril, S-cilazapril, S-trandolapril , S-pentopril and S-perindopril-in terms of sensitivity, selectivity, limit of detection, working concentration range, lifetime, response time, accura cy and precision.