Expression of the constitutive and inducible forms of heat shock protein 70 in human proximal tubule cells exposed to heat, sodium arsenite, and CdCl2

We determined the expression of the constitutive (hsc 70) and inducible (hs p 70) forms of heat shock protein 70 mRNA and protein in human proxima tubu le (HPT) cells exposed to lethal and sublethal concentrations of Cd+2 under both acute and attended conditions of exposure. The HPT cells exhibited th e classic heat shock response when subjected to a physical (heat) or chemic al stress (sodium arsenite); hsc 70 mRNA and protein levels were constant o r slightly increased, whereas hsp 70 mRNA and protein were greatly elevated . Acute exposure to 53.4 mu M CdCl2 for 4 hr failed to increase either hsc 70 mRNA or protein, a finding similar to that observed under classic condit ions of stress. However, under identical conditions of acute exposure to Cd 2+, the expected increase in hsp 70 protein level was suppressed as compare d to that found under classic conditions of physical or chemical stress. Th e decrease in hsp 70 protein level correlated to the reduced expression of mRNA from the hsp 70B gene. The expression of mRNA from the hsp 70A and hsp 70C genes was similar to that found when the cells were treated with heat shock or sodium arsenite. We modeled an extended exposure to Cd2+ by treati ng the cells continuously with Cd2+ at both lethal and sublethal levels ove r a 16-day time course. Chronic exposure to Cd2+ failed to increase either hsc 70 mRNA or protein levels in the HPT cells at a nonlethal dosage level and decreased hsc 70 mRNA and protein levels late in the time course of let hal exposure. Under identical conditions, the expression of hsp 70 protein remained at basal levels that were only marginally detectable throughout th e time course. Hsp 70A and hsp 70C mRNA levels were unaltered by extended e xposure to Cd2+, and hsp 70B mRNA was not detected during the 16-day time c ourse. Cd2+ is a poor inducer of hsc 70 and hsp 70 in the proximal tubule u nder both acute and long-term exposure. These results reinforce the fact th at the expression of hsp 70 protein does not result from the transcription of a single gene, but is derived from what may be a complex interplay of se veral underlying genes.