Structural elucidation of the O-antigenic polysaccharides from Escherichiacoli O21 and the enteroaggregative Escherichia coli strain 105

Citation
M. Staaf et al., Structural elucidation of the O-antigenic polysaccharides from Escherichiacoli O21 and the enteroaggregative Escherichia coli strain 105, EUR J BIOCH, 266(1), 1999, pp. 241-245
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
EUROPEAN JOURNAL OF BIOCHEMISTRY
ISSN journal
00142956 → ACNP
Volume
266
Issue
1
Year of publication
1999
Pages
241 - 245
Database
ISI
SICI code
0014-2956(199911)266:1<241:SEOTOP>2.0.ZU;2-4
Abstract
The structure of the O-antigen polysaccharide of the lipopolysaccharide fro m an enteroaggregative Escherichia coli (strain 105) has been elucidated, u sing primarily one-dimensional and two-dimensional NMR experiments. The seq uence of residues was deduced with heteronuclear multiple-bond correlation and NOESY experiments. The structure of the repeating unit of the polysacch aride from the enteroaggregative E. coli is as follows: [GRAPHICS] The structure of the O-antigen from enteroaggregative E. coli strain 105 wa s shown to be identical with that of E. coli O21 by sugar and methylation a nalyses as well as by H-1-NMR and C-13-NMR spectroscopy.