Occurrence of hordatines, the barley antifungal compounds, in a wheat-barley chromosome addition line

Hordatines A and B, strong antifungal components in shoots of barley seedli ngs, were found to occur in a barley chromosome 2H addition line to wheat, but not in a wheat line carrying the long arm of chromosome 2H, These resul ts showed that some genes that encode hordatine biosynthetic enzymes are lo cated on the short arm of chromosome 2H. The total content of the hordatine s was about six fold less than that in the parental barley, but was still a t a concentration more than 20-fold higher than that required for inhibitio n of spore germination of pathogenic fungi.