Prostaglandin E-1 protects against liver injury induced by Escherichia coli infection via a dominant Th2-like response of liver T cells in mice

Prostaglandin E series (PGEs) are known to protect against lipopolysacchari de (LPS)-induced liver injury by down-regulating the production of inflamma tory cytokines. We show here a novel mechanism whereby prostaglandin E-1 pr otects mice against liver injury after Escherichia coli infection. Prostagl andin E-1 administration suppressed circulating interleukin 12 (IL-12) leve ls but increased the IL-10 production after E. coli challenge. Furthermore, prostaglandin E-1-alpha-cyclodextrin (PGE(1)) shifted the Th1/Th2 balance of CD3(intermediate) IL-2R beta(+) T cells in the Liver to a dominant Th2-l ike response. Neutralization of endogenous IL-4 by administration of anti-I L-4 monoclonal antibody (mAb) diminished the inhibitory effect of prostagla ndin E-1 on liver injury after E. coli challenge. These results suggested t hat the Th2-like response of liver T cells may be at least partly involved in the mechanism whereby prostaglandin E-1 protects against E. coli-induced liver injury.