LKPNM: a prodrug-type ACE-inhibitory peptide derived from fish protein

It has been previously documented that the thermolysin-digest of "Katsuo-bu shi", a Japanese traditional food processed from dried bonito possesses pot ent inhibitory activity against angiotensin I-converting enzyme (ACE). The present authors isolated eight kinds of ACE-inhibitory peptides from it. Of these isolated peptides, LKPNM (IC50 = 2.4 mu M) was found to be hydrolyze d by ACE to produce LKP (IC50 = 0.32 mu M) with 8-fold higher ACE-inhibitor y activity relative to the parent peptide or LKPNM, suggesting that LKPNM c an be regarded as a prodrug-type ACE-inhibitory peptide. For assessment of relative antihypertensive activities of LKPNM and LKP to that of captopril, they were orally administered to SHR rats to monitor time-course changes o f blood pressures, whereby it was evidenced that both LKPNM and captopril s howed maximal decrease of blood pressure 4 h after oral administration and their efficacies lasted until 6 h post-administration. In sharp contrast, h owever, maximal reduction of blood pressure occurred as early as 2 h after administration of LKP. Minimum effective doses of LKPNM, LKP and captopril were 8, 2.25 and 1.25 mg/kg, respectively. When compared on molar basis, an tihypertensive activities of LKPNM and LKP accounted for 66% and 91% relati ve to that of captopril, respectively, whereas in vitro ACE-inhibitory acti vities of LKPNM and LKP were no more than 0.92% and 7.73% compared with tha t of captopril (IC50 = 0.022 mu M) It is of interest to note that both of t hese peptides exert remarkably higher antihypertensive activities in vivo d espite weaker in vitro ACE-inhibitory effects, which was ascertained by usi ng captopril as the reference drug. (C) 1999 Elsevier Science B.V. All righ ts reserved.