C. Holt et al., Some physico-chemical properties of nine commercial or semi-commercial whey protein concentrates, isolates and fractions, INT J FOOD, 34(5-6), 1999, pp. 587-601
Citations number
30
Categorie Soggetti
Food Science/Nutrition
Journal title
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
The physico-chemical properties are reported for a group of whey protein po
wders prepared on a commercial or semi-commercial scale by three companies
and chemically characterized as described elsewhere (Holt et al., 1999). Th
e dependence of the apparent beta-lactoglobulin % on the recovered % showed
that the nine samples could be placed in three distinct groups with beta-l
actoglobulin weight % of 70.9 +/- 1.1 (Group 1), 62.0 +/- 3.4 (Group 2) and
39.5 +/- 4.9 (Group 3). Measurements by H-1-NMR spectroscopy, on 3 of the
samples confirmed that the native fold still predominated in the beta-lacto
globulin. beta-lactoglobulin could be crystallized from all the powders and
the normal space group and cell dimensions were determined for the 8 sampl
es that gave crystals of good enough quality for X-ray studies. Differentia
l scanning microcalorimetry of samples dispersed in a phosphate buffer show
ed a clear difference between Groups 1 and 2 with a more prominent peak due
to alpha-lactalbumin in the Group 2 samples. Light scattering and size exc
lusion chromatography showed that two types of aggregates were present to a
variable extent in all the samples and after a heat treatment, the larger
aggregates tended to predominate in Group 2. The rheology measurements, als
o made in the phosphate buffer, showed a difference of gel stiffness during
heat treatment between the Group 1 and Group 2 samples with the exception
of the BORCwpc+ sample. Within each group, gel stiffness increased with the
degree of lactoslylation of the beta-lactoglobulin. Interfacial measuremen
ts on samples dispersed in water presented a more complex pattern of behavi
our although surface tension measurements at the air water interface of the
Group 2 samples showed a two-step pattern of surface tension decrease with
time, compared to a single step pattern in the Group 1 samples.