Cytopathic effect inhibition assay for determining the in-vitro susceptibility of herpes simplex virus to antiviral agents

Authors
Cotarelo, M Catalan, P Sanchez-Carrillo, C Menasalvas, A Cercenado, E Tenorio, A Bouza, E
Citation
M. Cotarelo et al., Cytopathic effect inhibition assay for determining the in-vitro susceptibility of herpes simplex virus to antiviral agents, J ANTIMICRO, 44(5), 1999, pp. 705-708
Citations number
9
Categorie Soggetti
Pharmacology,Microbiology
Journal title
Journal of antimicrobial chemotherapy
ISSN journal
03057453 → ACNP
Volume
44
Issue
5
Year of publication
1999
Pages
705 - 708
Database
ISI
SICI code
Abstract
We compare a rapid dilution method for the determination of antiviral susce ptibility of herpes simplex virus (HSV) with the plaque reduction assay, A total of 84 HSV clinical isolates were studied by both methods to detect in -vitro resistance to acyclovir and foscarnet, The rapid method showed for t he detection of HSV isolates resistant to acyclovir and foscarnet, a sensit ivity of 96.8% and 100% and specificity of 100% and 100%, respectively. Thi s method provides an easy and accurate screening procedure for the suscepti bility testing of HSV to antiviral agents.