Negative regulation of the gene for Fe-containing superoxide dismutase by an Ni-responsive factor in Streptomyces coelicolor

Citation
Hj. Chung et al., Negative regulation of the gene for Fe-containing superoxide dismutase by an Ni-responsive factor in Streptomyces coelicolor, J BACT, 181(23), 1999, pp. 7381-7384
Citations number
20
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
23
Year of publication
1999
Pages
7381 - 7384
Database
ISI
SICI code
0021-9193(199912)181:23<7381:NROTGF>2.0.ZU;2-K
Abstract
In Streptomyces coelicolor, transcription of the sodF genes, encoding Fe-co ntaining superoxide dismutases, is negatively regulated by nickel. Gel mobi lity shift assays,vith sodF1 promoter fragments and cell extracts from the A3(2) strain indicate the presence of a nickel-responsive DNA-binding prote in, most likely a transcriptional repressor. The boundary for the Ni-respon sive cis-acting region was identified both in vitro and vivo. Ni does not r egulate the level of the putative repressor but only the binding competence of this protein.