Hj. Chung et al., Negative regulation of the gene for Fe-containing superoxide dismutase by an Ni-responsive factor in Streptomyces coelicolor, J BACT, 181(23), 1999, pp. 7381-7384
In Streptomyces coelicolor, transcription of the sodF genes, encoding Fe-co
ntaining superoxide dismutases, is negatively regulated by nickel. Gel mobi
lity shift assays,vith sodF1 promoter fragments and cell extracts from the
A3(2) strain indicate the presence of a nickel-responsive DNA-binding prote
in, most likely a transcriptional repressor. The boundary for the Ni-respon
sive cis-acting region was identified both in vitro and vivo. Ni does not r
egulate the level of the putative repressor but only the binding competence
of this protein.