An improved transposon for the halophilic archaeon Haloarcula hispanica

Authors
Woods, WG Ngui, K Dyall-Smith, ML
Citation
Wg. Woods et al., An improved transposon for the halophilic archaeon Haloarcula hispanica, J BACT, 181(22), 1999, pp. 7140-7142
Citations number
19
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
22
Year of publication
1999
Pages
7140 - 7142
Database
ISI
SICI code
0021-9193(199911)181:22<7140:AITFTH>2.0.ZU;2-N
Abstract
An improved transposon (ThD73) for Haloarcula hispanica is described, Based on the halobacterial insertion sequence ISH28, it showed little target seq uence specificity but was biased toward a lower G+C content. Twenty randoml y selected ThD73 mutants were analyzed, and the DNA flanking their insertio ns revealed several recognizable sequences, including two (unrelated) ISH e lements.