The unvalidity of Kohlrausch' regulating function for Svensson's isoelectric focusing and stationary electrolysis at steady state

Kohlrausch' regulating function is of important significance in the field o f electrophoresis. In this paper, the relative regulating function is defin ed from Kohlrausch' regulating function. The relative values, including the limited values, of the regulating function for the stationary electrolysis of salt, on which the classic isoelectric focusing (IEF) is based, are com puted and compared with the computer program of the QBASIC written by us. T he results directly demonstrate that, (1) in a few cases the regulating fun ction is valid for the stationary electrolysis and IEF, whereas (2) the fun ction is, in most of cases, not valid for the stationary electrolysis and I EF at steady-state. Those findings may be useful for the studies on the rel ationships between Kohlrausch' regulating function and IEF and for the clas sification of numerous electrophoretic techniques. (C) 1999 Published by El sevier Science B.V. All rights reserved.