Rapid identification of Campylobacter, Arcobacter, and Helicobacter isolates by PCR-restriction fragment length polymorphism analysis of the 16S rRNAgene

Authors
Marshall, SM Melito, PL Woodward, DL Johnson, WM Rodgers, FG Mulvey, MR
Citation
Sm. Marshall et al., Rapid identification of Campylobacter, Arcobacter, and Helicobacter isolates by PCR-restriction fragment length polymorphism analysis of the 16S rRNAgene, J CLIN MICR, 37(12), 1999, pp. 4158-4160
Citations number
17
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
12
Year of publication
1999
Pages
4158 - 4160
Database
ISI
SICI code
0095-1137(199912)37:12<4158:RIOCAA>2.0.ZU;2-4
Abstract
A rapid two-step identification scheme based on PCR-restriction fragment le ngth polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter , and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were s uccessfully identified by this assay.