Development and evaluation of a rapid dipstick assay for serodiagnosis of acute human brucellosis

Authors
Smits, HL Basahi, MA Diaz, R Marrodan, T Douglas, JT Rocha, A Veerman, J Zheludkov, MM Witte, OWM de Jong, J Gussenhoven, GC Goris, MGA van der Hoorn, MAWG
Citation
Hl. Smits et al., Development and evaluation of a rapid dipstick assay for serodiagnosis of acute human brucellosis, J CLIN MICR, 37(12), 1999, pp. 4179-4182
Citations number
26
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
12
Year of publication
1999
Pages
4179 - 4182
Database
ISI
SICI code
0095-1137(199912)37:12<4179:DAEOAR>2.0.ZU;2-W
Abstract
A dipstick assay for the detection of brucella-specific immunoglobulin M an tibodies was evaluated with 707 sera from 247 laboratory-confirmed brucello sis patients and 342 control sera from brucellosis-free individuals. These sera were collected from six different countries. The assay was found to be highly sensitive and specific. In addition, the test is easy to use and do es not require specialized training or equipment, and the components are st able without a requirement for refrigeration. ALI of these factors make the test ideal for developing countries and rural settings.