Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively

Adenovirus (Ad) isolates from a large number of human immunodeficiency viru s (HN)-infected individuals were compared serologically and genetically wit h Ad isolates from immunocompetent patients. Between 1982 and 1994, stool a nd urine samples from 137 subjects with AIDS hospitalized in The Netherland s yielded 143 Ad strains. Forty additional Ad strains were obtained from 35 HIV-positive patients in Manchester, United Kingdom, in 1992 and 1993, Of these 183 HIV-associated Ad strains, 84% belonged to species D and 3% belon ged to species C, These strains were compared with 2,301 Ad strains collect ed during general diagnostic examinations in The Netherlands from 1973 to 1 992. Of the latter strains, 5% belonged to species D and 49% belonged to sp ecies C. Two of the Ads isolated from fecal specimens of AIDS patients repr esent new serotypes: candidate Ad serotype 50 (prototype strain, Wan) of su bspecies B1 and candidate Ad serotype 51 (prototype strain, Bom) of species D, The DNA restriction enzyme patterns of strains Wan and Bom differed fro m the patterns of all established prototypes.