Mu. Ahmed et al., Analysis of human rotavirus G serotype in Bangladesh by enzyme-linked immunosorbent assay and polymerase chain reaction, J DIARRH D, 17(1), 1999, pp. 22-27
Distribution of human rotavirus G serotype was investigated by enzyme-linke
d immunosorbent assay (ELISA) and polymerase chain reaction (PCR) with faec
al specimens obtained from children with diarrhoea in Bangladesh. By ELISA,
subgroup and G serotype were determined for 59.5% and 28.6% of group A rot
avirus-positive specimens respectively. However, of the 120 specimens, the
G serotype of which was not determined by ELISA, serotype of the 112 specim
ens was typed by PCR, In total, G serotype was assigned for 95.2% of all th
e specimens, showing the highest rate of G4 (41.7%), followed by G1 (23.2%)
and G2 (14.9%), Twenty-four specimens showed mixed types, such as G2 with
G1, G8 or G9, or G1 with G4, These results indicate that PCR combined with
ELISA is highly effective for G serotyping of rotavirus.