A kinetic study on the interaction between tazobactam (a penicillanic acidsulphone derivative) and active-site serine beta-lactamases

Authors
Perilli, M Franceschini, N Bonfiglio, G Segatore, B Stefani, S Nicoletti, G Perez, MDT Bianchi, C Zollo, A Amicosante, G
Citation
M. Perilli et al., A kinetic study on the interaction between tazobactam (a penicillanic acidsulphone derivative) and active-site serine beta-lactamases, J ENZ INHIB, 15(1), 1999, pp. 1-10
Citations number
18
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF ENZYME INHIBITION
ISSN journal
87555093 → ACNP
Volume
15
Issue
1
Year of publication
1999
Pages
1 - 10
Database
ISI
SICI code
8755-5093(1999)15:1<1:AKSOTI>2.0.ZU;2-E
Abstract
The interaction between tazobactam and several chromosome- and plasmid-enco ded (TEM, SHV, PSE types) class A and C beta-lactamases was studied by spec trophotometry. Tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as st part ner beta-lactam. A detailed kinetic analysis permitted measurement of the a cylation efficiency for some cephalosporinases and broad-spectrum beta-lact amases; the presence of a turn-over of acyl-enzyme complex was also evaluat ed.