Different cDNAs varying in the 3 '-untranslated end code for ceruloplasmin

Screening of a human Liver cDNA expression library with specific anticerulo plasmin antibodies allowed the identification of a 3' Eco RI cDNA fragment larger (+ 0.2 Kb) than the previously described cDNAs. Sequencing of this f ragment revealed that it possessed, respectively, 128 and 198 additional hp when compared to published data. PCR and hybridization experiments indicat ed that the additional 0.2 Kb piece belonged to the Cp gene. In addition, r esults from Southern hybridization of DNA digests and Northern hybridizatio n with mRNA from normal individuals, using this 0,2 Kb additional piece, su ggest that the Cp gene may be duplicated on chromosome 3. J. Trace Elem. Ex p. Med. 12:287-295, 1999. (C) 1999 Wiley-Liss. Inc.