STABILITY OF INVERTASE IN ALCOHOLYSIS REACTIONS WITH METHANOL

Authors
RODRIGUEZ M GOMEZ A GONZALEZ F BARZANA E LOPEZMUNGUIA A
Citation
M. Rodriguez et al., STABILITY OF INVERTASE IN ALCOHOLYSIS REACTIONS WITH METHANOL, Journal of molecular catalysis. B, Enzymatic, 2(6), 1997, pp. 299-306
Citations number
12
Categorie Soggetti
Chemistry Physical
Journal title
Journal of molecular catalysis. B, Enzymatic → ACNP
ISSN journal
13811177
Volume
2
Issue
6
Year of publication
1997
Pages
299 - 306
Database
ISI
SICI code
1381-1177(1997)2:6<299:SOIIAR>2.0.ZU;2-J
Abstract
The effect of methanol on the stability and structure of invertase is reported. It is shown by intrinsic fluorescence, that methanol induces irreversible changes in structure leading to its deactivation, while concentrations higher than 20% v/v also lead to aggregation and eventu al precipitation, a phenomenon which does not involve deactivation. On the other hand, low concentrations of methanol change the kinetic beh avior of enzyme as it acts as a mixed type inhibitor.