Chemiluminescence method for the determination of DNA using the Ru(bipy)(3)(2+)-Ce(IV) system

Authors
Han, HY Li, XY He, ZK Zeng, YE
Citation
Hy. Han et al., Chemiluminescence method for the determination of DNA using the Ru(bipy)(3)(2+)-Ce(IV) system, MIKROCH ACT, 132(1), 1999, pp. 105-109
Citations number
32
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
MIKROCHIMICA ACTA
ISSN journal
00263672 → ACNP
Volume
132
Issue
1
Year of publication
1999
Pages
105 - 109
Database
ISI
SICI code
0026-3672(1999)132:1<105:CMFTDO>2.0.ZU;2-M
Abstract
A direct chemiluminescence method for the determination of DNA has been dev eloped. The method is based on the chemiluminescence light produced by the reaction of DNA, Ru(bipy)(3)(2+)(bipy = 2,2'-bipydyl) and Ce(IV) in sulfuri c acid medium. Under the optimum conditions, the response is linearly propo rtional to the concentration of DNA between 7.0x10(-7) and 5.0x10-(5) g ml( -1). The final procedure allows the successful determination of DNA in thre e synthetic samples. This method is simple, rapid and specific.