Changes in choriocapillaris and retinal pigment epithelium in age-related macular degeneration

Retinal pigment epithelial cells (RPE) and the choriocapillaris are on oppo site sides of Bruch's membrane and control transport in and out of the reti na. In age-related macular degeneration (AMD), they may also be responsible for deposition of material in and on Bruch's membrane and the formation or regression of choroidal neovascularization (CNV). Indocyanine green (ICG) angiography can be used to visualize the choroidal vasculature and CNV. Fil ling of the choriocapillaris with ICG was delayed in subjects older than 50 years of age, and areas of hypofluorescence were observed in maculas of AM D subjects, often associated with CNV. Laser Doppler flowmetry of the chori ocapillaris in the macula demonstrated that choroidal blood flow and volume are reduced in subjects older than 46 years of age and further decreased i n subjects with AMD. The human choriocapillaris can be histologically studi ed in two dimensions by incubating the tissue for alkaline phosphatase acti vity, flat-embedding it in transparent polymer and sectioning it. Using thi s technique, choriocapillaris dropout was found to be associated with depos ition of material in Bruch's membrane in diabetic subjects. When RPE are re moved from Bruch's membrane, the choriocapillaris degenerates; the regenera tion of choriocapillaris can be blocked by Genistein, a tyrosine kinase inh ibitor. Finally, RPE cells may produce substances that both stimulate the f ormation and regression of CNV in animal models. These studies suggest that there may be a reduction in choriocapillaris flow in AMD, and this loss of choriocapillaris can be associated with the Bruch's membrane deposits that are hallmarks of AMD. Furthermore, RPE may stimulate the formation and reg ression of CNV and RPE loss can result in loss of choriocapillaris.