Os. De Peyer et al., A heterologous expression system for bovine lens transmembrane Main Intrinsic Protein (MIP) in Nicotiana tabacum plants, MOL VIS, 5(23), 1999, pp. NIL_1-NIL_5
We have developed a heterologous expression system for transmembrane lens m
ain intrinsic protein (MIP) in Nicotiana tabacum plant tissue. A native bov
ine MIP26 amplicon was subcloned into an expression cassette under the cont
rol of a constitutive Cauliflower Mosaic Virus promoter, also containing a
neomycin phosphotransferase operon. This cassette was transformed into Agro
bacterium tumefaciens by triparental mating and used to infect plant tissue
grown in culture. Recombinant plants were selected by their ability to gro
w and root on kanamycin-containing media. The presence of MIP in the plant
tissues was confirmed by PCR, RT-PCR and immunohistochemistry. A number of
benefits of this system for the study of MIP will be discussed, and also it
s application as a tool for the study of heterologously expressed proteins
in general.