Using countercurrent chromatography to assist in the purification of new annonaceous acetogenins from Annona squamosa

Three, new Annonaceous acetogenins, (2,4-cis and trans)-9-hydroxy asimicino ne (1), (2,4-cis and trans)-squamoxinone B (2) and (2,4-cis and traits) squ amoxinone C (3), were isolated from the bark of Annona squamosa with the he lp of countercurrent chromatography, Additionally found was isoannoreticuin (4), which is new to this species. Compound 1 is an adjacent bis-tetrahydr ofuran (THF) ring acetogenin with a hydroxyl group at the nine position and two more hydroxyls Banking the bis-THF system, while compounds 2-4 are all mono-THF ring acetogenins also possessing three hydroxyl groups. All four compounds are ketolactone-type acetogenins, and all show impressive cytotox icities in a panel of six human tumour cell Lines. Copyright (C) 1999 John Wiley & Sons, Ltd.