Epiphytic colonization of pear stigmas and hypanthia by bacteria during primary bloom

Pear blossoms were sampled during various stages of bloom in 1991 and 1992 from orchards at Cashmere, WA, and Corvallis and Medford, OR, for epiphytic populations of culturable bacteria. On stigmatic surfaces, bacteria were i solated from 2 to 32% of blossoms prior to petal expansion and from 47 to 9 4% of blossoms by petal fall. In general, a lower percentage of hypanthia t han stigmas supported bacterial populations. Randomly selected bacteria iso lated at population levels of greater than or equal to 10(4) CFU/tissue wer e identified by fatty acid methyl ester analysis. Diverse genera of gram-ne gative and -positive bacteria were identified from the Medford and Cashmere field sites. Pseudomonas syringae and Pseudomonas viridiflava were isolate d from all sites and were the predominant species detected at Corvallis, wh ere they were isolated from 28% of the blossoms sampled on a given date. Be cause most pear blossoms do not support detectable populations (greater tha n or equal to 10(2) CFU/tissue) of culturable bacteria prior to petal expan sion, we speculate that introduced biocontrol agents may become established with minimal competition from indigenous epiphytes at early bloom stages.