Silver-based crystalline nanoparticles, microbially fabricated

One mechanism of silver resistance in microorganisms is accumulation of the metal ions in the cell. Here, we report on the phenomenon of biosynthesis of silver-based single crystals with well-defined compositions and shapes, such as equilateral triangles and hexagons, in Pseudomonas stutzeri AG259. The crystals were up to 200 nm in size and were often located at the cell p oles. Transmission electron microscopy, quantitative energy-dispersive x-ra y analysis, and electron diffraction established that the crystals comprise at least three different types, found both in whole cells and thin section s. These Ag-containing crystals are embedded in the organic matrix of the b acteria. Their possible potential as organic-metal composites in thin film and surface coating technology is discussed.