Characterization of the molecular species of phosphatidylethanolamine fromkidney of the fresh water snail Lymneae stagnalis by mass spectrometry

The structural analysis of sixteen molecular species of diacyl glycerophosp hoethanolamine from fresh water snail Lymneae stagnalis kidney using chroma tography and mass spectrometry is described in this paper. 1-eicosadienoyl- 2-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine (PE 20:2-20:4), 1-eicos atrienoyl-2-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine (PE 20:5-20:2 ) and 1-eicosatrienoyl-2-eicosatetraenoyl-sn-glycero-2-phosphoethanolamine (PE 20:3-20:4) as well as 1-octadecanoyl-2-eicosatetraenoyl-sn-glycero-3-ph osphoethanolamine (PE 18:0-20:4), 1-ocetadecenoyl-2-eicosapentaneoyl-sn-gly cero-3-phosphoethanolamine (PE 18:1-20:4) and 1-octadecanoyl-2-eicosapentan eoyl-sn-glycero-3-phosphoethanolamine (PE 18:0-20:5) were found as the majo r molecular species, and the first three were tentatively identified as the novel species present in this biological material, The presence of a relat ively high content of 1,2-dieicosenoyl-sn-glycero-3-phosphoethanolamine spe cies (approximately 27% of total species) as well as the absence of 22-carb on fatty acid containing and plasmalogen PE molecular species are remarkabl e in healthy Lymneae stagnalis kidney. Copyright (C) 1999 John Wiley & Sons , Ltd.