Colony-promoting activity in mice kidneys with phenylhydrazine hemolytic anemia

Aqueous anemic mice kidney extracts (MKE) were assessed colony-promoting ac tivity (CPA) of hematopoietic progenitor cells in serum-free cultures stimu lated by interleukin-3 and erythropoietin (Epo) Mice with hemolytic anemia followed by phenylhydorazine (PHZ) injection for 3 days showed a decrease i n the hematocrit (25.4%) and an increase in serum Epo by 14-fold of the con trol on day 3 after the treatment At 3 clays, the total number of hematopoi etic progenitor cells in the bone marrow of PHZ mice decreased by 67% of th e control while these cells in the spleen increased to 22-fold of the contr ol on day 3 and 55-fold on day 6. A significant increase in CPA was observe d in MKE prepared from PHZ mice kidneys. Additionally, bone marrow suppress ive anemia induced by 5-fluorouracil resulted in enhanced CPA the same as f or PHZ mice, but in contrast anemia with suppression of Epo-production due to nephrotoxicity induced by cisplatin caused a decrease in CPA. These resu lts suggest that CPA in MKE correlates with hematopoietic conditions, and m ay have a definite role in hematopoiesis through the function of the kidney .